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The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm

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Mitochondrial bioregulation alters and coordinates the mitochondrial pools amount and quality between mitoses, allowing cellular mitochondrial activity regulation in response to cellular stress, metabolic status, and other intracellular hormonal and environmental signals and to induce mitophagy to complete the repair of diseased mitochondria to slow the progression of NAFLD