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March 2, 2021

The membrane was blocked with 5% skim milk for 2 hours at room temperature and probed with the following antibodies: -Tubulin (1:500, ab6046, Abcam), SIRT5 (1:1000, ab259967, Abcam), SIRT3 (1:1000, ab246522, Abcam), SIRT4 (1:1000, #24662, Cell Signaling Technologies), SIRT1 (1:1000, ab189494, Abcam), COX IV (1:2000, ABL1060, Abbkine Scientific Co., Ltd., Wuhan, Hubei, China), Cleaved-caspase1 (1:1000, 89332, Cell Signaling Technologies), NLRP3 (1:1000, ab263899, Abcam), GSDMD-N (1:1000, 50928, Cell Signaling Technologies), Collagen (1:1000, ab255809, Abcam), Collagen (1:1000, PA5-34787, Thermo Fisher Scientific), GSTP1 (1:1000, 15902-1-AP, ProteinTech), SPI1 (1:1000, #2258, Cell Signaling Technologies), and Malonyl-Lysine [Mal-Lys] MultiMab Rabbit mAb mix (1:1000, #14942, Cell Signaling Technologies) overnight at 4C

Speer, R

Several studies have provided sufficient evidence that miR-9 also participates in colorectal tumorigenesis acting as a tumor suppressor (112, 113)