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2024 Feb 28;9(10):11870-11882

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reported that ex vivo treatment of peripheral blood monocytes with methotrexate increased expression of IL-4 and IL-10 while IL-2 and interferon- expression were decreased, suggesting that the immunoregulatory role of methotrexate is also targeted at adjusting the balance between Th1 proinflammatory and Th2 anti-inflammatory cytokines [101]