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The beads were then washed three times using PBS, resuspended with 2 SDS-PAGE loading buffer and boiled for 10 min at 100 C

Free Radic Biol Med , 50: 874882, 2011

Numerous studies show a positive correlation between the level of TMAO concentration and the onset of several illnesses, including diabetes mellitus, hypertension, ischemic stroke, atrial fibrillation (AF), heart failure (HF), acute myocardial infarction (MI), and chronic kidney disease (CKD) [5]