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Determination of cell proliferation by measurement of 5-ethynyl-20-deoxyuridine (EdU) The 5-ethynyl-20-deoxyuridine (EdU-647) cell proliferation assay kit (Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 647 dye, C10340, ThermoFisher Scientific) was utilized to ascertain cell proliferation in blastocysts 27,32

Regulatory T cells that recognize a ubiquitous stress-inducible self-antigen are long-lived suppressors of autoimmune arthritis