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doi: 10.2174/1871530320666200613204426 [DOI] [PubMed] [Google Scholar] 82

After 16 h of incubation, the cells were collected, washed with PBS, and stained with 2,7-dichlorodihydrofluorescein diacetate (10 M, diluted in serum-free medium) at 37 C for 30 min

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(All committee members underwent the same ethics review and vetting process required of all FDA advisory committee members, Emily Hilliard, a spokesperson for the Department of Health and Human Services, told me in an email.) The decision is only a recommendation, and the FDA would still need to sign off on an actual rule change