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we express special thank to Qin-Qin Ruan, School of Art, Lanzhou University, for her guidance in the figures in this review

Samples were then washed three times with PBS for 5 min each, with DAPI (Invitrogen) being added to the second wash

The latter provide probes for structural determinants of the efficiency of disulfide pairing in the redox-coupled folding of nascent proinsulin (for review, see )