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This process not only impairs cellular energy metabolism but also exacerbates oxidative stress, further contributing to neuronal death (12,13)

Statistical analysis All quantitative results presented in this study were separately repeated to confirm the observations, with most of the experiments separately repeated three times or more

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Elevated cortisol levels affect gastrointestinal function through several mechanisms: inhibiting gut motility, reducing intestinal blood flow, impairing gut barrier integrity, altering gut microbiota composition, and increasing visceral hypersensitivity (Vanuytsel et al., 2014)