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Scaffold proteins of MAP-Kinase modules

This work was also supported by the Francis Crick Institute, which receives its core funding from Cancer Research UK (FC001223), the UK Medical Research Council (FC001223), and the Wellcome Trust (FC001223)

Answer: 4.9 In practice, most acidbase titrations are not monitored by recording the pH as a function of the amount of the strong acid or base solution used as the titrant

Briefly, plates were coated overnight with anti-human Fc (Jackson ImmunoResearch Laboratories 109-001-008), blocked for 2 h with PBS 2% BSA at RT, and incubated overnight at 4 C with recombinant Gp1-Fc protein derived from HEK293 cells transfected with LCMV glycoprotein vector