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The fluorescence intensity of SF650T-labeled Halo7-Rac1 released from the EVs in the endosomes into the cytoplasm in the recipient cells was measured as follows: puncta of the Halo7-Rac1, as Halo7-Rac1 remained in the EVs in the endosomes, were detected using Analyze Particles plugin of Image J as the particles with a threshold of more than 3000

Not only does this trigger the bodys antioxidant defenses to be produced, but the excess ROS can also escalate the production of the precursors for AGEs

NF-B, nuclear transcription factor B

For example, mitochondria isolated from the rat whole brain, when exposed to Aos, produce more reactive oxygen species (ROS) [86]