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Briefly, approximately 100 mg of tissue from the right lung was homogenized on ice utilizing an IKA ULTRA TURRAX T25 (disperser S25N-10G) in a redox quenching lysis buffer containing 5% trichloroacetic acid

While radiolabeled GSH has been extensively used to detect cellular uptake of GSH [5658], radiolabeling approaches can only provide a nonspecific readout of GSH metabolism based on the intracellular accumulation and/or proteome-wide incorporation of a specified radionuclide

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doi: 10.7326/0003-4819-137-12-200212170-00007 69 PangC.ZhengZ.ShiL.ShengY.WeiH.WangZ.et al