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10.1007/s11064-014-1333-z 116 LiS.OvermanJ

A 23 cm midline abdominal incision was made to expose the superior mesenteric artery (SMA), which was then clamped for 30 min

Membranes were incubated with primary antibodies overnight at 4 C, followed by incubation with secondary antibodies conjugated with either horseradish peroxidase or fluorescent dye