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The reads were processed and quality controlled using a malaria amplicon pipeline ( Markers with at least 10 coverage were considered to be successfully genotyped

Chromatographic separation was achieved using an Infinity Lab Poroshell 120 EC-C18 column (4.6 150 mm, 2.7 m particle size) and a binary gradient for the mobile phase (solvent A: water with 0.1% formic acid, and solvent B: acetonitrile with 0.1% formic acid)

Common mechanisms that play a role in developing GI disorders in patients with ASD include immune dysfunction, dietary metabolites, microbiota dysregulation, gut inflammation, dysautonomia and medication adverse reactions 12

Matrix stabilization maintains amorphous forms to prevent crystallization, while permeability enhancement occurs through membrane fluidity modulation