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As a result of this tight interplay between insulin action and cholesterol homeostasis, other studies have evidenced that IR may imprint profound disturbances in the profile of steroid hormones and bile acids [21, 49]

Unexpectedly, and in contrast to observations in fibroblasts, ACLY and ACSS2 double knockout (DKO) cancer cells remain viable and proliferate, maintain pools of cytosolic acetyl-CoA, and are competent to acetylate proteins in both cytosolic and nuclear compartments