glutathione sepharose 4 fast flow regeneration accelerates the cell cycle and cellular reprogramming in plant step glutathione S GSTrap™ Columns for Purification

Saitoh M, Nishitoh H, Fujii M, Takeda K, Tobiume K, Sawada Y, Kawabata M, Miyazono K, Ichijo H (1998) Mammalian thioredoxin is a direct inhibitor of apoptosis signal-regulating kinase (ASK) 1

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Thus, NLRP3-mediated pyroptosis and excessive autophagy in ovarian tissues of PCOS rats could be repressed by BSHLD treatment

Then cells were fixating by 4% paraformaldehyde, blocking by 1% bovine serum albumin (BSA), incubating with the primary antibody (Calreticulin Rabbit Monoclonal Antibody) at 4 C overnight, staining with the secondary antibody (Goat Anti-Rabbit IgG (Alexa Fluor@488)) for 1 h and DAPI for 10 min at room temperature