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Diluting the reconstituted solution with additional bacteriostatic water (30-90 units) reduces this irritation

Following a 24 h-treatment, cells were washed once with 1 PBS and incubated for 2 h in a saline buffer (139 mM NaCl, 0.56 mM MgCl 2 , 10 mM Hepes, 2.7 mM KCl, 1 mM K 2 HPO 4 , 1.8 mM CaCl 2 pH7.4 supplemented with 10 mM glucose) containing 100 M of 7-Azido-4-Methylcoumarin (AzMC) fluorescent probe (Sigma Aldrich), which selectively reacts with H 2 S to form a fluorescent compound

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