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To measure NMDA-induced inward current from GLP-1-receptor-positive neurons, we used magnesium-free aCSF (126 mM NaCl, 2.8 mM KCl, 2.5 mM CaCl 2 , 1.25 mM NaH 2 PO 4 , 26 mM NaHCO 3 and 5 mM glucose) containing 10 M CNQX and 100 M picrotoxin

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It is administered as part of a structured treatment plan by qualified professionals

Ann Pharmacother 35:10241027 Ramaekers J, Muntjewerff N, Van Veggel L, Uiterwijk M, OHanlon J (1998) Effects of nocturnal doses of mirtazapine and mianserin on sleep and on daytime psychomotor and driving performance in young, healthy volunteers