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The gut microbiota communicates with the brain by activating the HPA axis, altering neurotransmitters, producing microbial metabolites, influencing neurotrophic factors, modulating immune and inflammatory responses, and engaging vagus nerve pathways to regulate brain function and activity ( Lactobacillus , and probiotics can attenuate HPA axis activity, restore normal ACTH, CORT, and BDNF levels, and exert an antidepressant effect (Sun et al., 2022)

Distilled water was used throughout the experiment which was generated in-house

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