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Further research showed that it is competitive with the DNA substrate in methylation assays and alters the expression of methylated genes in MCF-7 breast cancer cells synergistically with 5-aza-2-deoxycytidine

The cells were grown in a defined medium containing DMEM/HAM F12, supplemented with 33 mM glucose, 2 mM glutamine, 100 U/ml penicillin, 100 g/ml streptomycin, 13 mM sodium bicarbonate, 5.5 g/ml transferrin, 10 g/ml insulin, 1 pM -estradiol, 3 nM triiodothyronine, 20 nM progesterone, 5 ng/ml sodium selenite, and 100 M putrescine

10.3402/fnr.v56i0.5329 Summary Keywords pulmonary fibrosis, vitamin, dietary nutrition, EMT, vitamin deficiency Citation Qu Y and Zhao Y (2025) Nutritional insights into pulmonary fibrosis: a comprehensive review on the impact of vitamins

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