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N-acetylcysteine as add-on to antidepressant medication in therapy refractory major depressive disorder patients with increased inflammatory activity: Study protocol of a double-blind randomized placebo-controlled trial

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After three washes in Tyrodes buffer (20 mM HEPES pH 7.4, 137 mM NaCl, 2.7 mM KCl, 1.8 mM CaCl 2 , 1 mM MgCl 2 , 0.4 mM NaH 2 PO 4 , 5.6 mM Glucose and 0.1% BSA), cells were either left untreated or incubated for the desired time with 50 ng/ml of DNP-HSA (Ag) (RBL cells) or 10 g/ml Rabbit anti human IgE (LAD-2 cells)

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