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Proteins were transferred to PVDF membranes (Thermofisher) using a Power BlotterSemi-dry Transfer System (Thermofisher) and incubated overnight with SHMT2 (E7F4Q) Rabbit mAb (Cell signaling, #3344) and vinculin Mouse mAb (Sigma-aldrich, #V9131) antibodies diluted in 5% skimmed milk in Tris-Buffered NaCl Solution with Tween 20 (TBST)

Mol Neurobiol 62 , 1545015477 (2025)

Cells were counted at 24 h, 48 h and 72 h of the treatment during medium change, and the old medium was collected each day and used for LDH assay

Interestingly, the PDXs that were previously found to be less responsive in vivo to induction chemotherapy, comprising vincristine, dexamethasone, and l-asparaginase (VXL, MLL-5, ALL-4, and ALL-19) were among the most sensitive to disulfiram (Fig