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doi: 10.1016/j.bbr.2016.06.051 155 SadekB.SaadA.SubramanianD.ShafiullahM.LazewskaD.Kiec-KononowiczcK

The eluted hFSP1 proteins were treated with Ulp1 to cleave off the 6his-SUMO tag, dialyzed against buffer A, re-loaded onto the Ni-Chelating Sepharose Fast Flow resin, and then the flow-through was concentrated and further purified by Superdex 200 Increase 10/300 GL columns (GE Healthcare, USA) with buffer B