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Yuichiro Yamada at Akita University for generously donating the tagged human GLP-1R cytomegalovirus promoter vector (pCMV)

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Cells were cultured and maintained DMEM/Ham's F-12 (Merck KGaA) supplemented with 10% FBS (Thermo Fisher Scientific, Inc.) and 100 g/ml streptomycin/100 units/ml penicillin (Thermo Fisher Scientific, Inc.) at 37C in a humidified atmosphere containing 5% CO2

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