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Most importantly, this cellular trafficking of gut-primed immune cells to secondary lymphoid organs and the synovial tissues was prevented by short-term zonulin antagonist treatment (Fig

This helps researchers overcome common experimental challenges with confidence

Cell cycle analysis 5-Ethynyl-2-deoxyuridine (EdU) incorporation assay was performed by using Click-iT Plus EdU Flow Cytometry Assay Kits (Thermo Fisher Scientific Inc.) following the manufacturers protocol