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Cultures were maintained in a humidified atmosphere of 5% CO 2 at 37C for 24 h and then incubated for the next 24-h period in DMEM-10% HS additionally containing recombinant HGF that was treated with peroxynitrite with or without trisulfide pre-treatment (see Figure 1d,f)

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However, it is important to note that the composition of breast milk changes throughout the lactation period, with colostrum being produced in the first 5 days, followed by transitional milk (615 days), and finally mature milk (15 days until weaning) [4, 14]