Human Genome-Wide CRISPRi Dual-sgRNA Library DriverMap Targeted RNA Sequencing It covers virtually all genes

It covers virtually all genes in the human genome

250 ug is enough for ~12 10-cm2 plates to make ~1-2 x 10^7 TU

and TRDs are shown

SVRTTATB-P Dox-On | No RFP Sensor All-in-One Dox-On Cloning Vector (pTMONBla-TRE-MCS-EFS-rtTA-2A-Blast)

Human Genome-Wide CRISPRi Dual-sgRNA Library DriverMap Targeted RNA Sequencing It covers virtually all genesPooled library of ~105,000 dual sgRNA constructs targeting ~19,000 human protein coding genes. Each construct expresses two sgRNA targeting the same gene. Designed to work with deactivated spCas9 (dCas9) complexed with the KRAB repressor to inactivate or down regulate gene expression. Five CRISPRi dual sgRNA constructs target each gene. Designs based on Horlbeck, et al., eLife 2016; 5: e19760 DOI: 10. 7554). Manual and Product Documentation Manual: