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Then, the sections were washed and incubated with secondary Alexa 488-conjugated anti-rabbit IgG or Alexa 568-conjugated anti-mouse IgG (Thermo Fisher Scientific, Waltham, MA)

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"What we know about nicotine's impact on the brain is concerning very concerning because we know that 96 percent of adult smokers started before the age of 21." According to the Campaign for Tobacco-Free Kids, each day 350 children under the age of 18 become daily-use smokers