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Spontaneously active DA neurons were identified based on previously established electrophysiological criteria 66,67 Fluorescence immunohistochemistry After euthanasia, induced by IP injection of euthasol (0.1 mL per 30 g at 150 mg/kg) or by paraformaldehyde (PFA) intracardiac perfusion, brains were rapidly removed and fixed in 4% PFA