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Eligible subjects were recruited from local vape shops, databases of people who previously smoked who attended a local smoking cessation center (Centro per la Prevenzione e Cura del Tabagismo (CPCT), University of Catania) and stopped smoking by switching to ECs, databases of people who previously smoked who took part in CoEHAR (CoEHAR, University of Catania) sponsored tobacco harm reduction and switching studies, social networks, WhatsApp chat of undergraduates and postgraduates of the University of Catania, and word of mouth among relatives and friends of study participants

However, some patients with proctitis or left-sided colitis may have a cecal inflammation patch

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Participants ranged in age from 18 to 80 with a mean of 36.4 and a standard deviation of 11