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MAI solid medium was placed in dark with controlled temperature (22C - 24C), relative humidity (75% - 90%)

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"While menthol usage is declining with all cigarette usage, it's declining at a much slower rate, about half the rate, I believe, than regular-flavored cigarettes." And because menthol, a mint-flavored additive, is popular among African-Americans, the companies focus ads and coupons there

The nicotine patch is the most commonly used [6] form of NRT but is less effective in heavy smokers ( 20 cigarettes/day) presumably due to inadequate replacement of nicotine to treat withdrawal, suggesting that higher doses may be more effective [14, 15]