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Glycerol also tested negative in the hypoxanthine-guanine phosphoribosyl transferase mutagenicity assay, sister chromatid exchange assay in Chinese hamster ovary cells, and unscheduled DNA synthesis in rat hepatocytes, at concentrations up to 1 mg/ml (CIR, 2015)

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A recent consensus report from the National Academies of Science, Engineering, and Medicine on the Public Health Consequences of E-cigarettes 7 showed moderate evidence that e-cigarettes increase coughing, wheezing as well as asthma exacerbation in adolescents