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obtusifolia de novo genome assembly, Illumina paired-end reads from the previous study were quality-filtered using Trimmomatic v0.39 with the parameters SLIDINGWINDOW:4:15 MINLEN:36, which were then used to construct the k-mer frequency-based histogram (k-mer = 31) using Jellyfish v2.2.10, and to estimate the genomic characteristics using GenomeScope v2 (Supplementary Figure 3) 18,19,20