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The membranes were blocked overnight in 5% skimmed milk and incubated for 1 h at room temperature with primary antibodies at dilutions of 1:3000(iNOS/eNOS, BD Transduction Laboratories, San Diego, CA, USA)

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The choice of materials is quite complex in order to satisfy the demands of long-term stable operation and high-speed production: High-strength alloys steel Excellent high-strength alloy steel makes up most of it