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For example, if your mom and dad had more pigmentation around the eyes or problems with circulation (both of which can cause bruised-looking eyes), its likely that your dark circles are hereditary

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doi: 10.1016/j.jacc.2016.03.569

Colony formation assay In brief, 100 cancer cells per well were plated in six-well plates in triplicates and cultured with nicotine (1 M) or CM from human primary neutrophils treated with nicotine or HDN/LDN CM from mouse primary neutrophils for 7 days as indicated before staining viable colonies with 0.5% crystal violet solution (Sigma-Aldrich)