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The much-touted filters intended to trap tar also captured nicotine, making filtered products less satisfying and causing smokers to compensate by taking more puffs, longer puffs, or more vigorous puffs (inhaling more deeply) to get their preferred dose of nicotine

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High nicotine content can cause side effects for users with low tolerance, and potential side effects include nausea, dizziness, and, in rare cases, nicotine overdose risks

The role of the YSL (Yellow Stripe-Like) proteins, which mediate the transport of metals in the form of chelates, has also been indicated as contributing to the efficient accumulation of Zn and Cd in the shoots/leaves of tobacco (Hodoshima et al