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This approach was selected as the preferred methodology for low-incidence events to reduce bias

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Furthermore, expression of SIRT1, a type of histone/protein deacetylases [162], was suppressed by cigarette smoke in inflammatory cells of murine lungs as well as macrophage cell lines, resulting in abrogation of the interaction of SIRT1 with RelA/p65 and acceleration of RelA/p65 acetylation [163]