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Accumulating evidence from the USA, UK and China has consistently reported the benefits of smoking cessation in reducing total and major cause mortality [12, 13]

Nitrogen sources in cigar tobacco leaves include protein, amino acid, nicotine and other nitrogen-containing compounds, among which nicotine is a unique nitrogen source and carbon source in cigar tobacco leaves, and is the core nutritional factor driving the assembly of nicotine-degrading microbial communities (Li et al., 2021)