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Preparation of standard solutions 1 mg/ml standard solutions were used to prepare stock solutions (1 ng/ml to 20 ng/ml) in LCMS grade methanol through serial dilutions and were stored at 4 C until further usage

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Although the risk of e-cigarettes for HNC has not yet been fully quantified, existing evidence has outlined a clear threat profile: the mechanisms of carcinogen release and tissue damage may elevate the incidence of HNC in the next 10 to 20 years

The results showed that treatment with water-soluble cholesterol increased ROS and secretion of inflammatory factors in HBE cells