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The libraries were further quantified on a Qubit 2.0 fluorometer using Qubit dsDNA HS kit (Life Technologies, USA) and by quantitative PCR (qPCR) using KAPA SYBR FAST qPCR Master mix and Illumina standards and primer premix (KAPA Biosystems, Wilmington, MA, USA), following the Illumina-suggested protocol

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According to research by American electronic cigarette company Juul Labs, there was a chance to shift 50 percent of adult consumers purchasing Juul products in shops from combustible cigarettes to Juul products

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