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Extracellular flux assayglycolysis rate assay Cells were plated at a density of 7500 cells/well in XF96 micro-plates (Agilent Technologies) in DMEM/F12 supplemented with 10% FBS 48 h prior to the assay and cultivated in a humified incubator at 37 C with 5% CO 2

The land use mix for each meshblock area unit [68] in the Taranaki region was then calculated using the entropy index described by Mavoa et al., [69] allowing local-level land use mix relevant to walkability to be examined