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After exposure, rats were anesthetized by intramuscular administration of ketamine with xylazine at a dose of 200 mg ketamine/kg + 10 mg of xylazine/kg and blood was collected from the heart ventricle at six-time intervals (0.25, 0.5, 1, 2, 3, 5 h) with three rats per time points Nicotine and its metabolites: cotinine, trans-3'-hydroxycotinine, nornicotine, nicotine N-oxide, and cotinine N-oxide, were determined in the collected plasma

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