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After cleavage with His-TEV to remove the His-MBP tag, the eluted fraction (SNX1-His-SUMO-SNX2a) was further loaded onto a Ni-NTA column and washed with buffer containing 20 mM HEPES (pH 7.4), 200 mM NaCl, and 40 mM imidazole to remove unbound samples

Persistent bleeding may lead to anaemia, especially if the episodes are frequent or heavy, and this can cause fatigue, shortness of breath, and general weakness

Over time, as more data and outlier sightings become available, Sells and her team will be able to measure the accuracy of their findings