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In the model 2, which adjusts for age and race, the total testosterone was increased by 0.09 (0.05, 0.13) for each additional unit of serum cotinine

Immunostaining of HBE cells ALI cultures were fixed in 4% paraformaldehyde 20 min at RT, followed by incubation in permeabilization solution (0.3% Triton X-100/PBS) for 15 min and in blocking solution (5% FBS/PBS/3% BSA) for 1 h at RT, incubated in primary antibodies overnight at 4 C, washed, incubated in secondary antibodies for 2 h at RT, washed, and then mounted for imaging

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In November 2019, the month before the age adjustment, Nevadas violation rate was 19.6% dangerously close to the federal threshold for noncompliance