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The precipitated pellet was then mixed with sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) sample loading buffer and analyzed by immunoblotting

As already mentioned, biologically active endotoxin present in cigarette smoke could act as an adjuvant in airway sensitization through TLR4 signaling

Furthermore, 1 in 1000 children is seriously injured and 1 in 10 000 dies from related injuries.3 Children under 4 years of age are at the greatest risk of severe injury, and 79% of fatalities resulting from abuse occur in this age group (44% in children under 1 year of age).4 Despite the work being done by child protective services, there is still a clear tendency towards an increase in the prevalence of child abuse