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J Clin Endocrinol Metab 102(5):16611672 Tarantini S et al (2017) Insulin-like growth factor 1 deficiency exacerbates hypertension-induced cerebral microhemorrhages in mice, mimicking the aging phenotype

Post-treatment, cell viability was then assessed by trypan blue exclusion assay using a Neubauer haemocytometer under an inverted bright- field microscope (Leica Microsystems, GmbH, Germany)