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WB analysis of extracts from OVCAR8 cells, transfected with 100 nM SignalSilence Control siRNA (Unconjugated) #6568 (-), SignalSilence KEAP1 siRNA I #5285 (+) or SignalSilence KEAP1 siRNA II #5289 (+), using recombinant monoclonal antibody KEAP1 (D6B12) Rabbit mAb #8047 (upper) or -Tubulin (11H10) Rabbit mAb #2125 (lower)

The activity of an enzyme can be influenced by the availability of cofactors, the concentration of substrates, the oxidative status, post-translational modifications or by inhibitors