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1, 5 ml of the biomass described in Section 2.2.1 was placed in a 2 ml screw cap tube along with 0.5 mm diameter glass beads with a weight ratio of 1:5 (glass beads/yeast suspension)

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In a subsequent reaction, LinA catalyses a second dehydrochlorination, and removes an additional HCl moiety from -PCCH to produce 1,3,4,6-tetrachloro-1,4-cyclohexadiene (1,4-TCDN)