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Description

In flour packed in paper bags and stored for 6 months, folic acid losses of 1520% at 25 C and 2022% at 40 C during storage at 65% relative humidity and those of 2227% at 25 C and 4753% at 40 C during storage at 85% relative humidity were observed

The data consistently shows measurable changes beginning around week 4 and becoming significant by week 8 to 12

After incubation, samples were clarified by centrifugation (16,000g, 20 min, 4 C) to remove large aggregates, and the supernatant fraction was used in downstream assays