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How to Store Bac Water Properly Temperature, light, and storage conditions Official labeling for bacteriostatic water lists storage at 20 to 25C, which is controlled room temperature

Reagent Caspase-3 (#9662, 1:1000), Cleaved caspase-3 (#9661, 1:1000), PARP (#9542, 1:1000), Bcl-xl (#2762, 1:1000), Bcl-2 (#2876, 1:1000), Bad (#9292, 1:1000), Bax (#2774, 1:1000), ATG-5 (#12994, 1:1000), ATG-7 (#8558, 1:1000), LC3A/B (#12741, 1:1000), Akt (#9272, 1:1000), p-Akt (Thr308) (#9275, 1:1000), p-mTOR (#2971, 1:1000), p-p70S6K (Thr389) (#9234, 1:1000) and -actin (#4970, 1:1000) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA)

Briefly, yeast cells from the second round of sorting and displaying parental scFv R023, were stained with 10 nM biotinylated soto-p 7 /A*11 for 30 min, followed by the removal of unbound antigen and subsequent incubation with 100 nM non-biotinylated competitor (non-biotinylated soto-p 7 /A*03) for 2 h at room temperature