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PC-CAR T cells can recognize peptides across HLAs Given the prerequisite of antigen processing and presentation necessary for detection of a given MHC peptide by immunopeptidomics, we proposed that identical peptides could be presented on additional HLA allotypes capable of binding anchor residues of a peptide

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Mass cytometry Heavy-metal conjugation of antibodies Antibodies were conjugated to heavy-metal ions with commercially available Maxpar (Fluidigm) or MIBItag (Ionpath) reagents according to manufacturers instructions, and as previously described 30 (see Supplementary Data 2)

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